BeStSel - Protein Circular Dichroism Spectra Analysis

								References: NEW Kardos et al. Protein Sci. 34:e70066 (2025), Micsonai et al. Nucleic Acids Res. 50:W90-98 (2022), Micsonai et al. Front. Mol. Biosci. 863141 (2022), Micsonai et al. Methods in Mol. Biol. vol 2199 (2021), Micsonai et al. Nucleic Acids Res. 46:W315-22 (2018), Micsonai et al. PNAS 112:E3095-103 (2015)

BeStSel (Beta Structure Selection) is a freely accessible webserver for secondary structure determination and fold recognition from protein circular dichroism spectra.

		Single spectrum analysis
		Secondary structure determination distinguishing parallel beta-sheets and antiparallel beta-sheets of different twists, and fold recognition.

		Secondary structure from PDB files
		Eight components of BeStSel and for comparison, DSSP and SELCON3 decomposition are calculated from the PDB file.

		Multiple spectra analysis
		Analysis of a series of spectra as a function of temperature, time, ligand concentration, etc.

		Extinction coefficient calculation
		Calculation of extinction coefficients at 205 nm and at 214 nm based on amino acid sequence and number of disulfide bonds.

		Fold recognition
		Prediction of fold class, architecture, topology and homology for the provided secondary structure contents.

		Disordered-Ordered Classification
		Binary disorder-order classification by analysing far-UV CD data.

		Thermal denaturation analysis
		Analysis of thermal denaturation curve (CD values in a given wavelength) with a two-state model.

		Cited by...
		Find articles of interest in which BeStSel has been used.

BeStSel™ (2014-2025) – ELTE Eötvös Loránd University, Budapest, Hungary

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Title: (optional)
BeStSel TUTORIAL
(PDF, 2.1 MB)

File input (text format):

OR paste data here:

Input format:
Please enter two data columns, wavelength and CD data. Separator can be space, tab, comma or semicolon. Please use dot as decimal point.
Example:

175   -0.47423
176   -0.35859
177   -0.25112
...

Sample spectrum (in delta epsilon)

Input units:

Concentration (microM)

Number of residues:

Pathlength (cm):


Title: (optional)	BeStSel TUTORIAL (PDF, 2.1 MB)
File input (text format):
OR paste data here:
	Input format: Please enter two data columns, wavelength and CD data. Separator can be space, tab, comma or semicolon. Please use dot as decimal point. Example: 175 -0.47423 176 -0.35859 177 -0.25112 ... Sample spectrum (in delta epsilon)
Input units:

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